4:18 PM
Nov 16, 2018
 |  BC Extra  |  Preclinical News

Spotlight co-founder uses CRISPR screen to ID T cell regulator genes for cancer

Spotlight Therapeutics Inc. (Hayward, Calif.) co-founder Alexander Marson and colleagues at University of California San Francisco developed a CRISPR-based screen to identify genes that control T cell replication and immunosuppression, and used the results to design a cancer immunotherapy with improved efficacy.

In a paper published Thursday in Cell, the researchers describe a hybrid screen combining lentiviral delivery of single guide RNAs with CRISPR-associated protein 9 (Cas9) protein electroporation (SLICE) to enable a genome-wide loss-of-function screen in primary human T cells. SLICE...

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