Optimized Cas9 nickases for prime editing, Amunix’s masked T cell engagers and more

Researchers from Singapore’s  Institute for Basic Science reported in Nature Communications a Cas9 nickase (nCas9) engineered to induce single-stranded DNA breaks for prime editing, while reducing the frequency of off-target double-strand breaks compared with commonly used nCas9 variants.

When incorporated into a prime editing system, the engineered nCas9 variant increased the frequency of correct edits without increasing the frequency of indels. The authors hold a patent on the tech...