ARTICLE | Tools & Techniques

Shotgun Resequencing

June 5, 2008 7:00 AM UTC

The complete sequencing of a plethora of genomes has spurred the development and marketing of new DNA resequencing technologies aimed at identifying genetic variations underlying disease and other aberrant phenotypes. However, the cost and complexity of such technologies has limited their use.1

Karolinska Institute researchers now suggest shotgun sequencing by hybridization (shotgun SBH) as a potentially cost-effective and accurate resequencing technique compared with marketed methods.2However, like all other current resequencing techniques, this method relies on the pre-existence of a reference genome sequence; therefore, shotgun SBH will not resolve the issue of how to quickly and cost-effectively perform de novogenome sequencing...