ARTICLE | Tools & Techniques

Degradation from within

February 20, 2014 8:00 AM UTC

Cornell University researchers have devised a way to selectively destroy intracellular proteins of interest using ubiquibodies-engineered molecules coupled to an enzyme that marks targets for degradation by the proteasome.1 The technique could be useful for screening the effects of knocking down targets that cannot be readily hit by siRNA.

Team leader Matthew DeLisa, a professor of engineering at Cornell, said that ubiquibodies are a type of intracellular antibody-a transgenic antibody fragment expressed within a cell to block the activity of a target protein.2...