Licensing status

Publication and contact information

Drug platforms

Polar body transfer in oocytes for nuclear DNA replacement without mitochondrial DNA

Polar body transfer could be used to replace nuclear DNA in an enucleated oocyte without also transferring mitochondrial DNA, which could help prevent mitochondrial diseases. Pronuclear transfer, spindle-chromosome transfer or polar body transfer was used to introduce genomic material into enucleated oocytes. After in vitro fertilization and transfer to female mice, oocytes generated from the three methods yielded viable pups at comparable rates. In tissue samples from these pups, high throughput DNA sequencing showed that polar body transfer led to the lowest levels of mitochondrial DNA carryover from the donor, whereas pronuclear transfer led to the highest DNA carryover. Next steps include conducting polar body transfer using human oocytes or embryos donated by subjects undergoing in vitro fertilization and isolating embryonic stem cells to evaluate carryover of donor mitochondria.

SciBX 7(28); doi:10.1038/scibx.2014.845
Published online July 24, 2014

Patent and licensing status undisclosed

Wang, T. et al. Cell; published online June 19, 2014;
Contact: Jianhong Zhu, Fudan University, Shanghai, China
Contact: Hongying Sha, same affiliation as above