Thursday, July 24, 2014
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Polar body transfer in
oocytes for nuclear DNA replacement without mitochondrial DNA
Polar body transfer could
be used to replace nuclear DNA in an enucleated oocyte without also
transferring mitochondrial DNA, which could help prevent mitochondrial
diseases. Pronuclear transfer, spindle-chromosome transfer or polar body
transfer was used to introduce genomic material into enucleated oocytes.
After in vitro fertilization and transfer to female mice, oocytes
generated from the three methods yielded viable pups at comparable rates. In
tissue samples from these pups, high throughput DNA sequencing showed that
polar body transfer led to the lowest levels of mitochondrial DNA carryover
from the donor, whereas pronuclear transfer led to the highest DNA carryover.
Next steps include conducting polar body transfer using human oocytes or
embryos donated by subjects undergoing in vitro fertilization and
isolating embryonic stem cells to evaluate carryover of donor mitochondria.
SciBX 7(28); doi:10.1038/scibx.2014.845
Published online July 24, 2014
Patent and licensing status
Wang, T. et al. Cell;
published online June 19, 2014;
Contact: Jianhong Zhu, Fudan University, Shanghai, China
Contact: Hongying Sha, same affiliation as above
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