Thursday, April 17, 2014
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Assays & screens
A refined, allele-specific
quantitative PCR assay called Intplex for analysis of circulating cell-free
DNA (cfDNA) mutations
Intplex could be used to
characterize small, highly fragmented, tumor-derived cfDNA for diagnostic
applications. Current cfDNA sequencing methods have difficulty determining
the presence of point mutations in fragments smaller than 100 base pairs. In
tumor tissue samples from patients with metastatic colorectal cancer, tumor
tissue analysis methods detected K-Ras
mutations in 36 of 95 samples and the BRAF
V600E mutation in 5 of 95 samples. In plasma samples from the same
patients, Intplex detected KRAS mutations in 37 of 95 samples and BRAF
V600E mutations in 5 of 95 samples, giving a concordance of 96% and
100%. Ongoing work includes using Intplex to characterize samples from larger
Published online April 17, 2014
licensed to an undisclosed company
Thierry, A.R. et al.
Nat. Med.; published online March 23, 2014;
Contact: Alain R. Thierry, Montpellier Cancer Research
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