Thursday, March 20, 2014
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antibodies for enhanced detection of dimerized or phosphorylated oncoproteins
Cell culture studies
suggest bispecific antibodies specific for dimerized or phosphorylated
proteins could be used in cancer diagnostics. In cultured, ligand-stimulated
human breast cancer cells, antibodies targeting HER2
growth factor receptor 3 (EGFR3;
that were fused by peptide or DNA linkers led to staining of HER2-HER3
dimers, whereas unlinked, monovalent antibodies did not. In formalin-fixed,
paraffin-embedded breast cancer cells, an antibody recognizing both a
phosphorylated and an unphosphorylated epitope of HER3 increased detection
sensitivity and decreased cross-reactivity compared with monovalent
antibodies. Next steps include further optimizing assay sensitivity and
applicability to human tissue samples and extending the assay to additional
Published online March 20, 2014
Patent applications filed;
available for licensing
van Dieck, J. et al.
Chem. Biol.; published online Feb. 13, 2014;
Contact: Michael Tacke, Roche Diagnostics GmbH, Penzberg, Germany
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