Thursday, March 20, 2014
Publication and contact
antibodies for enhanced detection of dimerized or phosphorylated oncoproteins
Cell culture studies
suggest bispecific antibodies specific for dimerized or phosphorylated
proteins could be used in cancer diagnostics. In cultured, ligand-stimulated
human breast cancer cells, antibodies targeting HER2
growth factor receptor 3 (EGFR3;
that were fused by peptide or DNA linkers led to staining of HER2-HER3
dimers, whereas unlinked, monovalent antibodies did not. In formalin-fixed,
paraffin-embedded breast cancer cells, an antibody recognizing both a
phosphorylated and an unphosphorylated epitope of HER3 increased detection
sensitivity and decreased cross-reactivity compared with monovalent
antibodies. Next steps include further optimizing assay sensitivity and
applicability to human tissue samples and extending the assay to additional
Published online March 20, 2014
Patent applications filed;
available for licensing
van Dieck, J. et al.
Chem. Biol.; published online Feb. 13, 2014;
Contact: Michael Tacke, Roche Diagnostics GmbH, Penzberg, Germany
All contents Copyright © 1993-2016 BioCentury Publications, Inc. ALL RIGHTS RESERVED. All use of this Web Site and its contents is governed by the BioCentury User Agreement
. The contents of this Web Site are protected under U.S. and foreign copyright and intellectual property laws, and no part of this Web Site or its contents may be photocopied, reproduced or retransmitted in any form without the written consent of BioCentury, which may be requested from Reprints/Permissions
. BioCentury®; The Bernstein Report on BioBusiness™; The BioCentury 100™; The Clear Route to ROI™; Because Real Intelligence is Hard to Find™; BCIQ™; and BioPharma's Knowledge Center™; are trademarks of BioCentury Publications, Inc., P.O. Box 1246, San Carlos, CA 94070. SciBX® and SciBX: Science-Business eXchange® are trademarks of Nature America, Inc. that are jointly used by BioCentury Publications, Inc. and Nature America, Inc.
Thanks for purchasing this article.
Please bookmark this url for future reference.
We have also sent you a confirming e-mail with a receipt and this same url for your
close [ X ]