Thursday, March 6, 2014
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Assays & screens
parallel RNA single-cell sequencing framework (MARS-Seq) for analyzing
transcriptional states in thousands of single cells
In vitro studies suggest MARS-Seq can be applied to
understand tissue heterogeneity in disease. Fluorescence-activated cell
sorting was used to pool homogenous cell populations. RNA sequencing on the
pooled samples yielded transcriptional profiles for 200-1,500 RNA molecules
per cell, and unsupervised clustering distinguished heterogeneous
transcriptional profiles. In a proof-of-concept application, the method
distinguished between B cells, NK cells, macrophages, monocytes, plasmacytoid
dendritic cells and other subtypes of dendritic cells derived from 4,000
mouse spleen cells. Next steps include improving RNA extraction efficiency
and increasing multiplexing capacity from 1,600 to 10,000 cells per
Published online March 6, 2014
Patent filed; available for
licensing from the Weizmann Institute of Science
through Yeda Research and Development Company Ltd.
Jaitin, D.A. et al.
Science; published online Feb. 14, 2014;
Contact: Ido Amit, Weizmann Institute of Science, Rehovot,
Contact: Amos Tanay, same affiliation as above
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