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Naïve human stem cell medium for maintenance of human induced pluripotent stem (iPS) cells in a naïve state

In vitro studies identified culture conditions to maintain human iPS cells in a naïve state. Culture of primed human iPS cells, embryonic stem cells (ESCs) or blastocyst-derived cells in naïve human stem cell medium generated pluripotent stem cells with features of naïve mouse iPS cells including a preinactivation X chromosome state, upregulation of pluripotency genes and downregulation of lineage commitment genes, and it produced decreases in DNA methylation compared with culture of primed human iPS cells alone. Mouse embryos injected with human naïve iPS cells and grown in female mice showed chimerism and incorporation of the human cells during organogenesis. Next steps include assessing properties of cells derived from naïve iPS cells (see Naïve human iPS cells, page 8).

SciBX 6(45); doi:10.1038/scibx.2013.1305
Published online Nov. 21, 2013

Patent application filed; available for licensing

Gafni, O. et al. Nature;
published online Oct. 30, 2013;
Contact: Jacob H. Hanna, Weizmann Institute of Science, Rehovot, Israel
Contact: Noa Novershtern,
same affiliation as above
Contact: Rada Massarwa,
same affiliation as above