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Single lipid vesicle assay to measure hydrolysis of disease-associated phospholipids

An assay using single vesicles to measure phospholipid hydrolysis could be used to identify and quantify disease-associated biomarkers. In the assay, single vesicles containing fluorescently labeled phospholipids were incubated with phospholipase A2 (PLA2) and enzyme activity was quantified by fluorescence microscopy. In cerebrospinal fluid samples from two patients with Alzheimer's disease (AD) and two healthy controls, PLA2 concentrations varied by up to 56%, but PLA2 activity varied by only 7%, suggesting that although enzyme levels may be higher in patients with AD, enzyme activity is not. Next steps include developing an advanced data analysis strategy that will help guide biomarker discovery.

SciBX 6(37); doi:10.1038/scibx.2013.1048
Published online Sept. 26, 2013

Patent application filed covering a device to perform this analytical method; not yet available for licensing; additional information available from GU Holding AB
Contact: Svante Hojer, GU Holding AB, Gothenburg, Sweden

Tabaei, S.R. et al. J. Am. Chem. Soc.; published online Aug. 19, 2013;
Contact: Fredrik Höök, Chalmers University of Technology, Gothenburg, Sweden