Thursday, September 26, 2013
This week in techniques
Publication and contact
Single lipid vesicle assay
to measure hydrolysis of disease-associated phospholipids
An assay using single
vesicles to measure phospholipid hydrolysis could be used to identify and
quantify disease-associated biomarkers. In the assay, single vesicles
containing fluorescently labeled phospholipids were incubated with phospholipase
and enzyme activity was quantified by fluorescence microscopy. In
cerebrospinal fluid samples from two patients with Alzheimer's disease (AD)
and two healthy controls, PLA2 concentrations varied by up to 56%,
but PLA2 activity varied by only 7%, suggesting that although
enzyme levels may be higher in patients with AD, enzyme activity is not. Next
steps include developing an advanced data analysis strategy that will help
guide biomarker discovery.
Published online Sept. 26, 2013
Patent application filed
covering a device to perform this analytical method; not yet available for
licensing; additional information available from GU
Contact: Svante Hojer, GU Holding AB,
Tabaei, S.R. et al. J.
Am. Chem. Soc.; published online Aug. 19, 2013;
Contact: Fredrik Höök, Chalmers University of Technology, Gothenburg,
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