Thursday, September 12, 2013
This week in
Publication and contact
Enhancing specificity of
RNA-guided clustered, regularly interspaced short palindromic repeats
(CRISPR)-based genome editing with paired guide RNAs
Enhancing the specificity
of CRISPR-based genome editing systems could improve its use in therapeutic
applications. CRISPR systems use small guide RNAs that pair with a target DNA
sequence and the CRISPR-associated protein 9 (Cas9) to excise target DNA. To
improve specificity, a system was designed that uses a Cas9 mutant enzyme
plus a pair of guide RNAs to excise a targeted DNA sequence. In cell lines,
this CRISPR-based editing system decreased off-target DNA excision by 50- to
1,000-fold compared with systems that use single guide RNAs. Next steps
include whole-genome sequencing studies to better characterize the
specificity of the Cas9 mutant and further improving the efficiency of the
system (see Crisper results for CRISPR, page 9).
SciBX 6(35); doi:10.1038/scibx.2013.978
Published online Sept. 12, 2013
Patent application filed;
available for licensing from the Broad
Institute of MIT and Harvard Office of Strategic Alliances
Ran, F.A. et al. Cell;
Aug. 29, 2013;
Contact: Feng Zhang, Broad Institute of MIT and Harvard, Cambridge,
All contents Copyright © 1993-2015 BioCentury Publications, Inc. ALL RIGHTS RESERVED. All use of this Web Site and its contents is governed by the BioCentury User Agreement
. The contents of this Web Site are protected under U.S. and foreign copyright and intellectual property laws, and no part of this Web Site or its contents may be photocopied, reproduced or retransmitted in any form without the written consent of BioCentury, which may be requested from Reprints/Permissions
. BioCentury®; The Bernstein Report on BioBusiness™; The BioCentury 100™; The Clear Route to ROI™; Because Real Intelligence is Hard to Find™; BCIQ™; and BioPharma's Knowledge Center™; are trademarks of BioCentury Publications, Inc., P.O. Box 1246, San Carlos, CA 94070. SciBX® and SciBX: Science-Business eXchange® are trademarks of Nature America, Inc. that are jointly used by BioCentury Publications, Inc. and Nature America, Inc.
Thanks for purchasing this article.
Please bookmark this url for future reference.
We have also sent you a confirming e-mail with a receipt and this same url for your
close [ X ]