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Clustered, regularly interspaced short palindromic repeats (CRISPR)-based editing system for one-step production of multigene knockout mice

CRISPR-based genome editing could be used to rapidly generate mice with multiple genetic mutations. Injection of one-cell mouse embryos with different concentrations of mRNA encoding CRISPR-associated protein 9 (Cas9) along with guide RNAs targeting two independent genes led to disruption of all four alleles 50%-90% of the time. In mouse embryonic stem cells, the CRISPR-based system was capable of disrupting up to five genes at once. Next steps include further optimizing the efficiency of the approach and determining whole-genome specificity.

SciBX 6(22); doi:10.1038/scibx.2013.555
Published online June 6, 2013

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Wang, H. et al. Cell; published online May 2, 2013;
Contact: Rudolf Jaenisch, Whitehead Institute for Biomedical Research, Cambridge, Mass.