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Assays & screens

Mutant methionyl-tRNA synthetase for protein labeling

Expression of a mutant methionyl-tRNA synthetase derived from Escherichia coli could be useful for producing modified variants of therapeutic proteins. The mutant E. coli methionyl-tRNA synthetase was expressed in a human cell line and designed to incorporate azidonorleucine at the N-terminus of proteins. In the same human cell line, supplementation with azidonorleucine led to the synthesis of azidonorleucine-labeled proteins that were detectable with mass spectrometry. Next steps could include generating therapeutic proteins that are fluorescently labeled or have improved pharmacological properties.

SciBX 6(12); doi:10.1038/scibx.2013.296
Published online March 28, 2013

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Ngo, J.T. et al. Proc. Natl. Acad. Sci.
USA
; published online
March 11, 2013;
doi:10.1073/pnas.1216375110
Contact: David A. Tirrell, California Institute of Technology, Pasadena, Calif.
e-mail:

tirrell@caltech.edu