Thursday, March 28, 2013
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Assays & screens
synthetase for protein labeling
Expression of a mutant
methionyl-tRNA synthetase derived from Escherichia coli could be
useful for producing modified variants of therapeutic proteins. The mutant E.
coli methionyl-tRNA synthetase was expressed in a human cell line and
designed to incorporate azidonorleucine at the N-terminus of
proteins. In the same human cell line, supplementation with azidonorleucine
led to the synthesis of azidonorleucine-labeled proteins that were detectable
with mass spectrometry. Next steps could include generating therapeutic
proteins that are fluorescently labeled or have improved pharmacological
Published online March 28, 2013
Patent and licensing status
Ngo, J.T. et al. Proc.
Natl. Acad. Sci.
USA; published online
March 11, 2013;
Contact: David A. Tirrell, California Institute of
Technology, Pasadena, Calif.
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