Thursday, February 14, 2013
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Assays & screens
Sensitizing bacteria to
antibiotics by increasing reactive oxygen species (ROS)
In silico prediction and in vitro screening could help
identify targets that increase bacterial ROS production and improve the
efficacy of marketed antibiotics. A workflow was developed that included
computational modeling of the metabolic state of Escherichia coli
followed by in vitro validation of gene targets that could modulate
ROS production. In vitro, knockout strains predicted to have increased
ROS production showed greater sensitivity to the generic antibiotics ampicillin
than control strains. Next steps could include using this approach to screen
for ROS-inducing compounds to use in combination with antibiotics.
Published online Feb. 14, 2013
Patent application filed;
licensed to EnBiotix Inc.
Brynildsen, M.P. et al.
Nat. Biotechnol.; published online
Jan. 6, 2013;
Contact: James J. Collins, Boston University, Boston, Mass.
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