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Assays & screens

Sensitizing bacteria to antibiotics by increasing reactive oxygen species (ROS) production

In silico prediction and in vitro screening could help identify targets that increase bacterial ROS production and improve the efficacy of marketed antibiotics. A workflow was developed that included computational modeling of the metabolic state of Escherichia coli followed by in vitro validation of gene targets that could modulate ROS production. In vitro, knockout strains predicted to have increased ROS production showed greater sensitivity to the generic antibiotics ampicillin and ofloxacin than control strains. Next steps could include using this approach to screen for ROS-inducing compounds to use in combination with antibiotics.

SciBX 6(6); doi:10.1038/scibx.2013.148
Published online Feb. 14, 2013

Patent application filed; licensed to EnBiotix Inc.

Brynildsen, M.P. et al. Nat. Biotechnol.; published online
Jan. 6, 2013;
Contact: James J. Collins, Boston University, Boston, Mass.