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Clustered, regularly interspaced short palindromic repeats (CRISPR) RNA editing system to modify genomic DNA

Four independent teams developed CRISPR-derived genome editing systems that could be used to modify genomic DNA in diverse biological systems. CRISPR is a bacterial adaptive immunity system that uses host-expressed nucleases and RNA repeats to cleave foreign DNA. To adapt this system to cleave and edit genomic DNA, the four teams designed a DNA vector that expressed a bacteria-derived CRISPR-associated nuclease together with guide RNAs that contained CRISPR features and homology to host genes. Two teams used the system to induce site-specific insertions and deletions in multiple genomic loci in cultured mouse and human cells, whereas a third team developed a system to cleave and edit specific sites within the zebrafish genome. The fourth team engineered a system to modify endogenous genomes in two distinct bacterial species. Next steps include characterizing and optimizing the specificity of the approach.

SciBX 6(5); doi:10.1038/scibx.2013.125
Published online Feb. 7, 2013

Patent application filed for findings in first study; licensing status undisclosed

Findings in second study unpatented; licensing status not applicable

Patent application filed for findings in third study; licensing status undisclosed

 

Patent application filed for findings in fourth study; licensing status undisclosed

Jinek, M. et al. eLife; published online Jan. 29, 2013;
doi:10.7554/eLife.00471

Contact: Jennifer Doudna, University of California, Berkeley, Calif.
e-mail:
doudna@berkeley.edu

Hwang, W.Y. et al. Nat. Biotechnol.; published online Jan. 29, 2013;
doi:10.1038/nbt.2501
Contact: J. Keith Joung, Massachusetts General Hospital, Charlestown, Mass.
e-mail:
jjoung@partners.org
Contact: J.-R. Joanna Yeh, same affiliation as above
e-mail:
jyeh1@partners.org

Cho, S.W. et al. Nat. Biotechnol.; published online Jan. 29, 2013;
doi:10.1038/nbt.2507
Contact: Jin-Soo Kim, Seoul National University, Seoul, South Korea
e-mail:
jskim01@snu.ac.kr

Jiang, W. et al. Nat. Biotechnol.; published online Jan. 29, 2013;
doi:10.1038/nbt.2508
Contact: Luciano A. Marraffini, The Rockefeller University, New York, N.Y.
e-mail:
marraffini@rockefeller.edu
Contact: David Bikard, same affiliation as above
e-mail:
dbikard@rockefeller.edu