This week in techniques



Licensing status

Publication and
contact information

Assays & screens

High throughput sequencing strategy for pairs of immunoglobulin heavy (VH) and light (VL) variable regions

A method to sequence paired VH and VL regions could help improve the design of vaccines, therapeutics and diagnostics. Sequencing methods usually do not provide any information on which VH and VL chains are paired. The new approach involves depositing, lysing and capturing mRNA from single B cells in high-density microwell plates. This mRNA is reverse transcribed, amplified as a pair by linkage PCR and then sequenced. The strategy identified variable region pairs for three types of B cells and was faster, less expensive and able to identify more paired sequences than single-cell RT-PCR. Next steps include optimizing the approach to increase throughput (see Sequencing single B cells, page 9).

SciBX 6(5); doi:10.1038/scibx.2013.122
Published online Feb. 7, 2013

Patent application filed; licensing status available from The University of Texas at Austin

DeKosky, B.J. et al. Nat. Biotechnol.; published online Jan. 20, 2013;
Contact: George Georgiou, The University of Texas at Austin, Austin, Texas