Thursday, September 13, 2012
Publication and contact
High throughput flow
cytometry for small molecule screening
multiplexed flow cytometry could provide a way to rapidly screen small
molecules in immune cells. Samples of human peripheral blood mononuclear
cells (PBMCs) were labeled with different combinations of 7 unique lanthanide
isotopes and arrayed in a 96-well plate. Multiplexed flow cytometry was then
used to measure the response of PBMC intracellular signaling to 27 kinase
inhibitors under a variety of conditions. The method generated IC50
values and cellular phosphorylation levels associated with each inhibitor as
well as intracellular signaling networks activated by the inhibitors. Next
steps include using the method to screen for compounds that modulate
epigenetic and metabolic pathways in cancer cells.
The method used in the paper is marketed by DVS Sciences Inc. under the name CyTOF.
SciBX 5(36); doi:10.1038/scibx.2012.968
Published online Sept. 13, 2012
Flow cytometry method
covered by patents owned by DVS Sciences; licensing status undisclosed
B. et al. Nat. Biotechnol.; published online Aug. 19,
Contact: Gary P. Nolan, Stanford University School of Medicine,
Palo Alto, Calif.
All contents Copyright © 1993-2017 BioCentury Publications, Inc. ALL RIGHTS RESERVED. All use of this Web Site and its contents is governed by the BioCentury User Agreement
. The contents of this Web Site are protected under U.S. and foreign copyright and intellectual property laws, and no part of this Web Site or its contents may be photocopied, reproduced or retransmitted in any form without the written consent of BioCentury, which may be requested from Reprints/Permissions
. BioCentury®; The Bernstein Report on BioBusiness™; The BioCentury 100™; The Clear Route to ROI™; Because Real Intelligence is Hard to Find™; BCIQ™; and BioPharma's Knowledge Center™; are trademarks of BioCentury Publications, Inc., P.O. Box 1246, San Carlos, CA 94070. SciBX® and SciBX: Science-Business eXchange® are trademarks of Nature America, Inc. that are jointly used by BioCentury Publications, Inc. and Nature America, Inc.
Thanks for purchasing this article.
Please bookmark this url for future reference.
We have also sent you a confirming e-mail with a receipt and this same url for your
close [ X ]