Thursday, September 13, 2012
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High throughput flow
cytometry for small molecule screening
multiplexed flow cytometry could provide a way to rapidly screen small
molecules in immune cells. Samples of human peripheral blood mononuclear
cells (PBMCs) were labeled with different combinations of 7 unique lanthanide
isotopes and arrayed in a 96-well plate. Multiplexed flow cytometry was then
used to measure the response of PBMC intracellular signaling to 27 kinase
inhibitors under a variety of conditions. The method generated IC50
values and cellular phosphorylation levels associated with each inhibitor as
well as intracellular signaling networks activated by the inhibitors. Next
steps include using the method to screen for compounds that modulate
epigenetic and metabolic pathways in cancer cells.
The method used in the paper is marketed by DVS Sciences Inc. under the name CyTOF.
SciBX 5(36); doi:10.1038/scibx.2012.968
Published online Sept. 13, 2012
Flow cytometry method
covered by patents owned by DVS Sciences; licensing status undisclosed
B. et al. Nat. Biotechnol.; published online Aug. 19,
Contact: Gary P. Nolan, Stanford University School of Medicine,
Palo Alto, Calif.
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