Thursday, September 13, 2012
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Assays & screens
antibody-free quantification of proteins in biofluids
A mass spectrometry-based
method could be useful for quantifying proteins in biofluids with ELISA-like
sensitivity. In the method's first step, microliter volumes of
trypsin-digested human serum were fractionated via liquid chromatography-tandem
mass spectrometry (LC-MS/MS) into a 96-well plate. In the second step,
LC-MS/MS analysis of individual or multiple combined fractions detected
peptides corresponding to target proteins, thereby enabling quantification of
those proteins at low pg/mL concentrations. In human serum from patients with
prostate cancer, the method measured prostate-specific antigen (KLK3; PSA) concentrations with an
accuracy comparable to that of ELISA. Ongoing work includes using the
technique to quantify undisclosed proteins in human urine.
SciBX 5(36); doi:10.1038/scibx.2012.964
Published online Sept. 13, 2012
Unpatented; available for
Shi, T. et al. Proc. Natl. Acad.
Sci. USA; published online Sept. 4,
Contact: Wei-Jun Qian, Pacific Northwest National Laboratory,
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