Licensing status

Publication and contact information

Assays & screens

Mass spectrometric, antibody-free quantification of proteins in biofluids

A mass spectrometry-based method could be useful for quantifying proteins in biofluids with ELISA-like sensitivity. In the method's first step, microliter volumes of trypsin-digested human serum were fractionated via liquid chromatography-tandem mass spectrometry (LC-MS/MS) into a 96-well plate. In the second step, LC-MS/MS analysis of individual or multiple combined fractions detected peptides corresponding to target proteins, thereby enabling quantification of those proteins at low pg/mL concentrations. In human serum from patients with prostate cancer, the method measured prostate-specific antigen (KLK3; PSA) concentrations with an accuracy comparable to that of ELISA. Ongoing work includes using the technique to quantify undisclosed proteins in human urine.

SciBX 5(36); doi:10.1038/scibx.2012.964
Published online Sept. 13, 2012

Unpatented; available for partnering

Shi, T. et al. Proc. Natl. Acad. Sci. USA; published online Sept. 4, 2012;
Contact: Wei-Jun Qian, Pacific Northwest National Laboratory, Richland, Wash.