Monday, September 24, 2001
Methods used to engineer proteins with improved or novel functions by
recombining two genes of interest depend on greater than 70% sequence homology between the
two genes, a requirement that significantly limits the diversity of protein production.
Now researchers at Pennsylvania State University have described a method to generate
libraries with multiple recombinations independent of sequence homology.
"The method itself is unlimited and completely independent of
sequence identity," said Stefan Lutz, the primary author of the report last week in
the Proceedings of the National Academy of Sciences. As proof of this lack of
threshold, Lutz told