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Jun 04, 2007
 |  BioCentury  |  Tools & Techniques

Signal snapshots

Mass spectrometry-based proteomics can reveal protein-protein interactions using large sample sizes and analyzing binding relationships long after a cell signal has started. But it has been difficult to parse the molecular events as they occur through time in order to separate background binding from functionally important interactions.

Researchers from the University of Southern Denmark have come up with a simple way to analyze cell signaling events that occur as soon as one second after a ligand binds to its receptor and initiates the signal, a vast improvement on previous time interval analyses, which often use data from 30 seconds or more after signal initiation.

As published recently in Nature Biotechnology, the researchers coupled a conventional quench-flow system with mass spectrometry. Using this tool, they were able to take quantitative measurements of specific phosphopeptides - in this case the phosphorylated bits of epidermal growth factor receptor - at various...

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