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12:00 AM
 | 
Sep 28, 2009
 |  BioCentury  |  Emerging Company Profile

Helix: Running Hot and Cold

Traditional real-time PCR technologies use a Peltier heater to heat and cool a metal block to drive the PCR reactions, which is a slow process and can lead to inconsistent results. Helixis Inc. believes the fluid-based RT-PCR system it launched this month will reduce the inconsistency between wells and decrease the wait time between reactions. Improved consistency should increase the accuracy of results, particularly where diagnostics are concerned.

A single PCR cycle consists of a denaturation step followed by primer annealing and fragment extension steps. Denaturation occurs at 94-95 ˚C, while the annealing and extension steps occur at lower temperatures. Annealing temperatures have a...

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