PPL, Roslin Institute other research news

PPL and Roslin scientists produced 5 transgenic sheep using their nuclear transfer procedure (see BioCentury Feb. 24). Fibroblasts cultured from Poll Dorset sheep were transfected with a gene encoding a human plasma protein or a reporter gene. Transfected cells were then fused with enucleated sheep eggs. Scottish Blackface ewes were implanted with the fused transgenic eggs and carried the resulting embryos to term. ...