BioCentury
ARTICLE | Company News

Amersham International plc other research news

July 10, 1995 7:00 AM UTC

Harvard University researchers published in the Proceedings of the National Academy of Sciences a method for engineering novel DNA polymerases with enhanced properties for use in DNA sequence analysis.

Active site hybrids of DNA polymerases from E. coli and Thermus aquaticus were constructed, and changes in the polypeptide chain of the active site produced enhanced ability to incorporate dideoxynucleotides. This is particularly useful for automated DNA sequencing that uses fluorescently labeled dideoxynucleotides, because unincorporated nucleotides lead to a high fluorescent background, the researchers said. ...