1:41 PM
Jan 10, 2019
 |  BC Innovations  |  Translation in Brief

Guided design for CRISPR editing

How algorithm, set of target gene features could help predict results of CRISPR

Separate teams from the Wellcome Sanger Institute and The Francis Crick Institute have developed an algorithm and identified a set of genetic features, respectively, that could help drug developers improve the safety of CRISPR-Cas9-based therapies by predicting results of template-free gene editing.

In the absence of a template for homology-directed DNA repair, DNA breaks generated by CRISPR-associated protein 9 (Cas9) are repaired by either non-homologous end joining (NHEJ) or microhomology-mediated end joining (MMEJ). Both pathways are prone to error, which makes it challenging to predict editing outcomes and raises safety concerns.

While there are tools for predicting on-target activity and off-target effects of...

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