9:18 AM
 | 
Oct 04, 2018
 |  BC Innovations  |  Distillery Techniques

Assays and screens; biomarkers

TECHNOLOGY: Bioinformatics screens; cellular assays; gene profiling

A single-cell, sequencing-based method that correlates RNA expression with chromatin accessibility could help identify biomarkers of cellular activity and/or cell type. The method involves: distributing extracted cell nuclei into assay plate wells at 5,000 nuclei per well; reverse-transcribing the nuclear RNA and labeling the resulting cDNA with well-specific barcodes and molecule-specific sequences; cleaving the genomic DNA at accessible chromatin sites and labeling the fragments with...

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