BioCentury
ARTICLE | Preclinical News

Sequencing-based method identifies neoantigen-specific TCRs

November 12, 2018 11:00 PM UTC

In a Nature Biotechnology paper, researchers described a method to identify TCRs that bind to specific neoantigens, which could enable efficient and inexpensive production of personalized T cell therapies.

The high-throughput technique, developed by researchers at University of Texas at Austin and Shanghai Jiao Tong University and dubbed TetTCR-seq, mixes T cells with tetramer complexes of major histocompatibility complex (MHC) bound to antigens and linked to a fluorescently labeled DNA barcode. The neoantigen-bound T cells are then sorted via flow cytometry, and their TCRα and TCRβ chains are sequenced to identify the antigen-binding TCR...