ARTICLE | Product Development
A guide to serum virus neutralization assays
The what, how and why of serum virus neutralization assays in COVID-19 vaccine development
May 23, 2020 2:22 AM UTC
While the level of neutralizing antibody titers needed to protect against SARS-CoV-2 infection in humans remains an open question, the assays to test them will be an essential tool for COVID-19 vaccine developers.
The assay is performed by first collecting serum from an immunized animal and heat-inactivating it. The serum, which contains neutralizing antibodies, is then serially diluted, and various dilutions are mixed with either live virus or pseudovirus. ...