Cellular fluorescence assay to assess allosteric modulator potency in drug discovery

Karolinska, Pasteur and UCSD researchers have developed a cellular fluorescence assay that can efficiently screen for potent transcription factor allosteric modulators during lead identification.

Unlike compounds that work by binding targets’ active sites, allosteric modulators bind non-enzymatic sites and regulate protein function by changing their target’s shape, interactions with other proteins or subcellular location. ...