BioCentury
ARTICLE | Distillery Techniques

Techniques: Platelet derived growth factor AB (PDGFAB) and Vidaza azacitidine to reprogram terminally differentiated cells into induced multipotent stem cells f

May 5, 2016 7:00 AM UTC

Reprogramming terminally differentiated cells with PDGFAB and Vidaza azacitidine could help generate induced multipotent stem cells for regenerative therapies. Treatment with PDGFAB and Vidaza converted primary mouse osteocytes, primary mouse adipocytes and primary human adipocytes into multipotent stem cells that could differentiate into multiple cellular lineages, including bone, cartilage and fat. In a mouse model of spinal injury, transplants of mouse osteocytes pretreated with PDGFAB and Vidaza developed into new skeletal muscle cells, vascular endothelial cells, bone cells and cartilage cells, whereas transplants of non-pretreated mouse osteocytes or bone marrow mesenchymal stem cells did not develop into new cell types. Next steps include testing the effects of human induced multipotent stem cells in other animal models of tissue injury...