Approach

Summary

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Assays & screens

Lentivirus-based clustered, regularly interspaced short palindromic repeats (CRISPR) library for high throughput screening

A high throughput screening system using lentivirus-based CRISPR libraries could be used to identify therapeutic targets. A CRISPR library was constructed using lentiviral DNA to target 291 human genes with 3 different kinds of single guide RNAs (sgRNAs). In a functional screening study designed to identify genes associated with bacterial toxin resistance, known toxin targets as well as previously unknown genes were identified. In HeLa cells, knockdown of the newly identified genes caused toxin resistance. Next steps include expanding the size of the library and the number of sgRNAs targeting each gene.

SciBX 7(19); doi:10.1038/scibx.2014.567
Published online May 15, 2014

Patent application filed; unavailable for licensing

Zhou, Y. et al. Nature; published online April 9, 2014;
doi:10.1038/nature13166
Contact: Wensheng Wei, Peking University, Beijing, China
e-mail:

wswei@pku.edu.cn