Thursday, March 13, 2014
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Gene catalog for
differentiating human pluripotent stem cells (hPSCs) into mature,
insulin-producing pancreatic b cells
In vitro and computational studies identified genes that
could help develop strategies for b cell production
from hPSCs. Antibody staining for insulin followed by cell sorting was used
to isolate pure populations of insulin-producing cells derived from hPSCs.
Subsequent gene expression and hierarchical clustering analyses demonstrated
similarities between insulin-producing cells derived from different hPSCs.
The analyses also found similarities between hPSC-derived cells and fetal
insulin-producing cells and differences between hPSC-derived cells and adult
insulin-producing cells. Further comparison between fetal and adult cells
identified a catalog of genes responsible for b cell
maturation and metabolic functionalization. Next steps include identifying
the gene regulatory network associated with the catalog and potential drug
Published online March 13, 2014
Patent application filed
covering method for analyzing RNA following intracellular sorting (MARIS);
available for licensing
Hrvatin, S. et al. Proc.
Natl. Acad. Sci. USA; published online Feb. 10, 2014;
Contact: Douglas A. Melton, Harvard University, Cambridge, Mass.
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