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Disease models

Clustered, regularly interspaced short palindromic repeats (CRISPR) genome editing to produce genetically modified monkeys

Primate studies suggest CRISPR could be used to develop genetically modified monkeys that model disease. In single-cell cynomolgus monkey embryos, injection of a pool of five single guide RNAs (sgRNAs) targeting peroxisome proliferation-activated receptor-g (PPARG; PPARg), recombinant activating gene 1 (RAG1) and nuclear receptor subfamily 0 group B member 1 (NR0B1) plus CRISPR-associated 9 (Cas9) mRNA resulted in embryos with simultaneous disruptions in two target genes. In infant monkeys born after embryos were transferred to female monkeys, umbilical cord, placenta and ear puncture tissues from twin monkeys had the same genetic modifications in Pparg and Rag1. Next steps include developing germline-modified monkeys using the strategy.

SciBX 7(9); doi:10.1038/scibx.2014.268
Published online March 6, 2014

Findings unpatented; unavailable for licensing

Niu, Y. et al. Cell; published online Jan. 30, 2014;
doi:10.1016/j.cell.2014.01.027
Contact: Jiahao Sha, Nanjing Medical University, Nanjing, China
e-mail:

shajh@njmu.edu.cn
Contact: Xingxu Huang, Model Animal Research Center of Nanjing University, Nanjing, China
e-mail:

xingxuhuang@mail.nju.edu.cn
Contact: Weizhi Ji, Yunnan Key Laboratory of Primate Biomedical Research, Kunming, China
e-mail:

wji@kbimed.com