Approach

Summary

Licensing status

Publication and contact information

Drug platforms

Human pluripotent stem cell-derived lung and airway epithelial cells

Human pluripotent stem cell-derived lung and airway epithelial cells could be used to model respiratory diseases. In human pluripotent stem cells, a growth factor cocktail plus small molecule signaling inhibitors of bone morphogenetic protein (BMP), transforming growth factor-b (TGFB; TGFb) and porcupine-mediated wingless-type MMTV integration site (WNT) palmitoylation generated progenitor-like cells of the anterior foregut endoderm. In immunodeficient mice, progenitor-like cells transplanted under the kidney capsule differentiated into several types of lung and airway endodermal and mesodermal cells that expressed mature lung and airway epithelial markers. On a human lung matrix or fibronectin-coated plastic, differentiation of progenitor-like cells led to lung and airway endodermal and mesodermal cells that expressed all markers detected in the in vivo-differentiated cells. Next steps include using the system to model idiopathic pulmonary fibrosis (IPF).

SciBX 7(2); doi:10.1038/scibx.2014.71
Published online Jan. 16, 2014

Patent application filed; unlicensed

Huang, S.X.L. et al. Nat. Biotechnol.; published online Dec. 1, 2013;
doi:10.1038/nbt.2754
Contact: Hans-Willem Snoeck, Columbia University Medical Center, New York, N.Y.
e-mail:
hs2680@columbia.edu