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Transcription activator-like effector nucleases (TALENs) selectively targeting individual microRNAs

A library of TALEN-mediated miRNA knockouts could help study miRNA function. A library of TALEN pairs designed to disrupt 274 specific miRNA loci was designed and validated in human embryonic and leukemia cells. In breast cancer cells, TALEN pairs targeting miR-141 and miR-200C, which differ by one nucleotide in their target-selective seed regions, resulted in the upregulation of non-overlapping sets of target mRNAs and decreased cell proliferation rates compared with no TALENs. Next steps include using the TALEN library to selectively delete single miRNAs for research applications.

SciBX 6(47); doi:10.1038/scibx.2013.1368
Published online Dec. 12, 2013

Patent application filed for improved TALEN architecture; licensed by ToolGen Inc.; available for licensing

Kim, Y.-K. et al. Nat. Struct. Mol. Biol.; published online Nov. 10, 2013;
doi:10.1038/nsmb.2701
Contact: V. Narry Kim, Seoul National University, Seoul, South Korea
e-mail:
narrykim@snu.ac.kr

Contact: Jin-Soo Kim, same affiliation as above
e-mail:
jskim01@snu.ac.kr