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Assays & screens

Whole-exome sequencing to identify antigens for adoptively transferred, autologous tumor-infiltrating lymphocytes (TILs)

Whole-exome sequencing could help to identify mutated antigens to express in TILs for adoptive transfer immunotherapy. Whole-exome sequencing identified nonsilent, somatic mutations in the DNA of melanoma cells that were absent from that of matched normal cells. A peptide-major histocompatibility complex (MHC) binding algorithm was then used to select high-affinity candidate T cell epitopes that would be recognized by TILs. Top candidate epitopes were synthesized and screened for their ability to induce interferon-g (IFNG; IFN-g) release from TIL cell lines derived from three patients with melanoma. Next steps include evaluating the association between TIL recognition of mutant antigens and long-term tumor regression.

SciBX 6(21); doi:10.1038/scibx.2013.529
Published online May 30, 2013

Unpatented; licensing status not applicable

Robbins, P.F. et al. Nat. Med.; published online May 5, 2013;
doi:10.1038/nm.3161
Contact: Paul F. Robbins, National Institutes of Health, Bethesda, Md.
e-mail:

paulrobbins@mail.nih.gov