Thursday, April 18, 2013
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Generation of high-affinity
DNA aptamers using non-natural nucleotides
nucleotides could be useful for generating high-affinity DNA aptamers.
Libraries of DNA aptamers were generated using the four natural nucleotides
plus the non-natural nucleotide 7-(2-thienyl)imidazo[4,5-b]pyridine. In
vitro selection experiments against human VEGF-165
and interferon-g (IFNG;
IFN-g) identified aptamers that
bound to the proteins with subpicomolar and subnanomolar Kd
values that were a 100-fold improvement over DNA aptamers made from only
natural nucleotides. Next steps include using the technology to develop
high-affinity aptamers against small molecules and cells (see Expanding the aptamer
alphabet, page 7).
Published online April 18, 2013
Patent applications filed;
available for licensing from TagCyx Biotechnologies
Kimoto, M. et al. Nat.
Biotechnol.; published online April 7, 2013;
Contact: Ichiro Hirao, RIKEN Systems and Structural Biology
Center, Kanagawa, Japan
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