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Assays & screens

Panel of peptoid ligands of aquaporin-4 (AQP4) autoantibodies for diagnosing neuromyelitis optica (NMO)

A panel of peptoid ligands that bind AQP4 autoantibodies could be useful for diagnosing NMO. A library of 100,000 peptoid compounds was synthesized on beads and subsequently screened for their ability to bind AQP4 autoantibodies, which are a hallmark of NMO. The 10 lead hits from the screen were used to develop a fluorescence assay to analyze patient sera. In serum samples from healthy controls and patients who have NMO, the assay correctly identified 14 of the 15 samples that came from patients who have NMO and distinguished them from sera samples taken from patients with multiple sclerosis (MS), lupus, Alzheimer's disease (AD) or narcolepsy. Next steps include identifying peptoid ligands with increased affinity for AQP4 autoantibodies.

SciBX 6(14); doi:10.1038/scibx.2013.345
Published online April 11, 2013

Patented; licensing status undisclosed

Raveendra, B.L. et al. Chem. Biol.; published online March 21, 2013;
doi:10.1016/j.chembiol.2012.12.009
Contact: Thomas Kodadek, Scripps Florida, Jupiter, Fla.
e-mail:
kodadek@scripps.edu