Approach

Summary

Licensing status

Publication and contact information

Assays & screens

Class A b-lactamase (blaC)-specific detection of Mycobacterium tuberculosis

Fluorescent probes specific for M. tuberculosis blaC could be useful for detecting M. tuberculosis in clinical samples. Chemically modified cephalosporins that release a fluorophore when bound to blaC were synthesized. In vitro, the probes emitted a fluorescence signal and showed 1,000-fold greater selectivity for blaC over a closely related class A b-lactamase produced by other bacterial strains. In unprocessed patient sputum samples, the lead probe detected M. tuberculosis within 10 minutes even when fewer than 10 colony-forming units of bacilli were present. Global BioDiagnostics Corp. is conducting a proof-of-concept study on the probes and has begun development of a diagnostic to detect M. tuberculosis (see Probing for a point-of-care TB test, page 8).

SciBX 5(36); doi:10.1038/scibx.2012.962
Published online Sept. 13, 2012

Multiple patents pending; licensed to Global BioDiagnostics

Xie, H. et al. Nat. Chem.; published online Sept. 2, 2012;
doi:10.1038/nchem.1435
Contact: Jianghong Rao, Stanford University, Stanford, Calif.
e-mail:

jrao@stanford.edu