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Fluorescent reporter-expressing dengue virus

Fluorescent reporter-expressing dengue virus could be useful for screening and evaluating therapeutic candidates to treat the disease. A serotype-2 dengue virus was engineered to express GFP or firefly luciferase. In mice, fluorescence imaging showed that the engineered reporter virus primarily localized to lymphoid and gut tissues. An expression screening system using human hepatoma cells infected with the engineered virus showed that 12 interferon (IFN)-stimulated genes from a library of over 350 such genes inhibited viral replication. Next steps could include using the fluorescent reporter virus in studies to evaluate compounds that modulate IFN-stimulated genes.

SciBX 5(35); doi:10.1038/scibx.2012.939
Published online Sept. 6, 2012

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Schoggins, J.W. et al. Proc. Natl. Acad. Sci. USA; published online Aug. 20, 2012;
doi:10.1073/pnas.1212379109
Contact: Charles M. Rice, The Rockefeller University, New York, N.Y.
e-mail:
ricec@rockefeller.edu