Hi-fi hybridomas

A team from ETH Zurich has developed a method for producing high-fidelity hybridomas by using CRISPR-based gene editing, and believes the system can address the unreliability of research antibodies that has fed into the "reproducibility crisis." The result is a faster process and purer yield, according to the group.

Traditionally, hybridoma cells are made by immunizing animals against an antigen, isolating antibody-producing B cells and then individually fusing each of the B cells to a myeloma cell partner to create immortalized cell lines that secrete a single antibody with the relevant specificity. ...