Thursday, February 13, 2014
1. In vivo shRNA discovery of immunotherapy targets. T
cells infected with shRNA libraries were injected into mice with melanoma.
Several days later, T cells were purified from tumors and secondary lymphoid
tissue. Deep sequencing then identified shRNAs that enabled T cells to
preferentially accumulate and proliferate in tumors more so than in spleens.
(Figure based on Figure 1 in ref. 1.)