Thursday, December 12, 2013
Anti-miRNA delivery by B cells. Almanza
et al. have engineered B cells to deliver anti-microRNA to other immune
cells. The findings suggest a new avenue for delivering therapeutic miRNA.
A plasmid (a)
engineered to encode a short sequence complementary to microRNA-150 (anti-miR-150) is
transfected in murine
B cells (b). In cell culture and in vivo, engineered B cells
interact with T cells, which naturally express miR-150 (c). When B cells
and T cells interact to form an immunological synapse (d), anti-miR-150
is secreted into membrane-bound extracellular vesicles (exosomes), which are
absorbed by the T cells (e). This leads to degradation and knockdown of
endogenous miR-150 (f).