Thursday, July 24, 2014
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DNA sequencing of
drug-resistant mammalian cells and clustered, regularly interspaced short palindromic
genome editing to identify drug targets
Cell culture studies
suggest high-throughput sequencing and CRISPR-Cas9 genome editing can be
combined to rapidly validate drug targets. The approach uses high-throughput
sequencing to identify mutations in drug-resistant clones and a CRISPR-Cas9
genome editing approach called DrugTargetSeqR to confirm the roles of the
mutations in resistance. In cancer cell lines, the combination approach correctly
identified the targets for two compounds in clinical trials. In another human
cancer cell line, a related method correctly identified the targets and their
resistance-conferring mutations for two toxic compounds. Next steps include
automating the workflow of DrugTargetSeqR.
Published online July 24, 2014
For findings from both
studies, DrugTargetSeqR patented; available for licensing from The Rockefeller University
Office of Technology Transfer
Kasap, C. et al. Nat.
Chem. Biol.; published online June 15, 2014;
Contact: Tarun M. Kapoor, The Rockefeller University, New
Smurnyy, Y. et al. Nat. Chem. Biol.; published online June 15, 2014;
Contact: Yan Feng, Novartis Institutes for BioMedical
Research, Cambridge, Mass.
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