Thursday, June 5, 2014
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Recombination activating gene 2 (RAG2)
knockout pigs to model immunodeficiency and evaluate transplant-based
Pigs with RAG2
knocked out could be useful as models of human immunodeficiencies and for
testing transplant-based therapies. In vitro, engineered transcription
activator-like effector nucleases (TALENs)
were used to knock out RAG2 in pig fibroblasts, which were then used
in a somatic cell nuclear transfer procedure to generate eight RAG2
knockout pigs. The RAG2 knockout pigs failed to gain weight and showed
a severe combined immunodeficiency phenotype that resulted in death or
euthanasia before 29 days. A subsequent cohort of RAG2 knockout pigs
housed in cleaner conditions remained healthy for eight weeks and rapidly
developed teratomas when injected with human induced pluripotent stem (iPS)
cells. Next steps could include using the pigs to evaluate transplantation of
stem cell-derived tissues.
Published online June 5, 2014
Unpatented; pigs available
from the National Swine Resource and Research Center at the
University of Missouri
Lee, K. et al. Proc.
Natl. Acad. Sci. USA; published online May 5, 2014;
Contact: Jin-Hoi Kim, Konkuk University, Seoul, South Korea
Contact: Randall S. Prather, University of Missouri,
Contact: R. Michael Roberts, same affiliation as above
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