Thursday, May 8, 2014
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Assays & screens
Identification of small
molecule protein inhibitors with laser-assisted detection of specific binding
A laser-based system could
help identify new small molecule inhibitors of various proteins. The system
directs a laser at adjacent wells in a 384-well plate, with one well
containing a small molecule and immobilized target protein and the other well
containing only the small molecule. Specific small molecule-protein binding
resulted in a detectable difference between the wavelengths of light
reflected back to the system by photonic sensors in the two adjacent wells.
anhydrase II (CAII)
as the target protein, the system correctly distinguished the nanomolar CAII
from 47 other compounds that do not specifically bind CAII. Ongoing work has
increased the sensitivity of the system for small molecule-protein binding
and will be reported in a forthcoming publication.
Published online May 8, 2014
Patented; available for
Zhang, M. et al. J. Am.
Chem. Soc.; published online April 10, 2014;
Contact: Brian T. Cunningham, University of Illinois at
Urbana-Champaign, Urbana, Ill.
Contact: Paul J. Hergenrother, same affiliation as above
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