Thursday, March 13, 2014
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effector nuclease (TALEN)-mediated genome editing to generate genetically
Primate studies suggest
TALENs could be used to generate genetically modified monkeys to model
disease. In single-cell rhesus or cynomolgus monkey embryos, microinjection
of three methyl
CpG binding protein 2 (MECP2;
TALEN plasmids plus a RAD51
plasmid resulted in eight-cell-stage embryos with MECP2 disruptions.
Embryo transfer to female monkeys produced three aborted male fetuses and one
live female infant. Sequencing analysis of tissues from the TALEN monkeys
showed that the TALEN fetuses and infant had multiple point mutations across MECP2
and no off-target mutational effects. Next steps include using the system to
generate monkey models for neurological and metabolic diseases.
Published online March 13, 2014
status not applicable
Liu, H. et al. Cell Stem
Cell; published online Feb. 13, 2014;
Contact: Weizhi Ji, Yunnan Key Laboratory of Primate
Biomedical Research, Kunming, China
Contact: Yi Eve Sun, same affiliation as above
Contact: Siguang Li, Tongji University School of Medicine, Shanghai,
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