Thursday, March 6, 2014
Publication and contact
Induced pluripotent stem
(iPS) cell-derived immortalized megakaryocyte progenitor cell lines as a
self-renewing source for human platelets
In vitro and mouse studies suggest iPS cell-derived
platelets could be used for clinical applications. Stable, immortalized
megakaryocyte progenitor cell lines were generated from iPS cells by
overexpression of BMI1 polycomb ring finger oncogene
and c-Myc (MYC), and the cells
produced platelets when transgene expression was turned off. In vitro,
aggregation of immortalized megakaryocyte progenitor cell line-derived
platelets was lower than that of fresh human platelets but comparable to that
of other iPS cell-derived platelets or pooled human endogenous platelets. In
a mouse model of thrombocytopenia, the platelets adhered to laser-exposed
vessel walls and contributed to thrombus formation. Next steps could include
optimizing megakaryocyte maturation, efficiency of platelet release into
liquid culture systems and platelet yield per megakaryocyte.
Published online March 6, 2014
Patent application filed;
in negotiations for licensing to an undisclosed company; unavailable for
Nakamura, S. et al. Cell
Stem Cell; published online Feb. 13, 2014;
Contact: Koji Eto, Kyoto University, Kyoto, Japan
All contents Copyright © 1993-2015 BioCentury Publications, Inc. ALL RIGHTS RESERVED. All use of this Web Site and its contents is governed by the BioCentury User Agreement
. The contents of this Web Site are protected under U.S. and foreign copyright and intellectual property laws, and no part of this Web Site or its contents may be photocopied, reproduced or retransmitted in any form without the written consent of BioCentury, which may be requested from Reprints/Permissions
. BioCentury®; The Bernstein Report on BioBusiness™; The BioCentury 100™; The Clear Route to ROI™; Because Real Intelligence is Hard to Find™; BCIQ™; and BioPharma's Knowledge Center™; are trademarks of BioCentury Publications, Inc., P.O. Box 1246, San Carlos, CA 94070. SciBX® and SciBX: Science-Business eXchange® are trademarks of Nature America, Inc. that are jointly used by BioCentury Publications, Inc. and Nature America, Inc.
Thanks for purchasing this article.
Please bookmark this url for future reference.
We have also sent you a confirming e-mail with a receipt and this same url for your
close [ X ]