Thursday, March 6, 2014
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Induced pluripotent stem
(iPS) cell-derived immortalized megakaryocyte progenitor cell lines as a
self-renewing source for human platelets
In vitro and mouse studies suggest iPS cell-derived
platelets could be used for clinical applications. Stable, immortalized
megakaryocyte progenitor cell lines were generated from iPS cells by
overexpression of BMI1 polycomb ring finger oncogene
and c-Myc (MYC), and the cells
produced platelets when transgene expression was turned off. In vitro,
aggregation of immortalized megakaryocyte progenitor cell line-derived
platelets was lower than that of fresh human platelets but comparable to that
of other iPS cell-derived platelets or pooled human endogenous platelets. In
a mouse model of thrombocytopenia, the platelets adhered to laser-exposed
vessel walls and contributed to thrombus formation. Next steps could include
optimizing megakaryocyte maturation, efficiency of platelet release into
liquid culture systems and platelet yield per megakaryocyte.
Published online March 6, 2014
Patent application filed;
in negotiations for licensing to an undisclosed company; unavailable for
Nakamura, S. et al. Cell
Stem Cell; published online Feb. 13, 2014;
Contact: Koji Eto, Kyoto University, Kyoto, Japan
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