Thursday, January 16, 2014
Publication and contact
Assays & screens
Coupled ligand affinity
capture and massively parallel DNA sequencing (Chem-seq) for genome-wide
mapping of interactions between small molecules and chromatin
Chem-seq can help map
interactions between small molecules and their protein targets in chromatin
of cells. In Chem-seq, cells are first treated with an affinity capture-capable
analog of a compound targeting a chromatin-bound protein. Next, the proteins
are cross-linked to DNA, and the associated DNA is purified and sequenced. In
proof-of-concept studies in cultured human multiple myeloma (MM) cells, a
biotinylated analog of the bromodomain inhibitor JQI
mapped the compound to the same DNA regions as chromatin immunoprecipitation
sequencing (ChIP-seq) assays with antibodies against bromodomain containing 4 (BRD4). Next steps include
using Chem-seq to investigate drugs that target proteins that control
transcription on a genome-wide scale and developing a more robust
second-generation version of Chem-seq.
Published online Jan. 16, 2014
Patent application filed;
licensed to Syros
Anders, L. et al. Nat.
Biotechnol.; published online Dec. 15, 2013;
Contact: James E. Bradner, Dana-Farber Cancer Institute,
Contact: Richard A. Young, Whitehead Institute for
Biomedical Research, Cambridge, Mass.
All contents Copyright © 1993-2016 BioCentury Publications, Inc. ALL RIGHTS RESERVED. All use of this Web Site and its contents is governed by the BioCentury User Agreement
. The contents of this Web Site are protected under U.S. and foreign copyright and intellectual property laws, and no part of this Web Site or its contents may be photocopied, reproduced or retransmitted in any form without the written consent of BioCentury, which may be requested from Reprints/Permissions
. BioCentury®; The Bernstein Report on BioBusiness™; The BioCentury 100™; The Clear Route to ROI™; Because Real Intelligence is Hard to Find™; BCIQ™; and BioPharma's Knowledge Center™; are trademarks of BioCentury Publications, Inc., P.O. Box 1246, San Carlos, CA 94070. SciBX® and SciBX: Science-Business eXchange® are trademarks of Nature America, Inc. that are jointly used by BioCentury Publications, Inc. and Nature America, Inc.
Thanks for purchasing this article.
Please bookmark this url for future reference.
We have also sent you a confirming e-mail with a receipt and this same url for your
close [ X ]