Thursday, November 21, 2013
This week in techniques
Publication and contact
Small molecule target
discovery using forward genetics
In vitro, cell culture and mouse studies suggest a high
hairpin RNA screening platform could be useful for identifying the
targets of lead compounds. A cell culture screen for small molecule
inhibitors of acute lymphoblastic leukemia growth identified STF-11804,
which inhibited tumor growth at nanomolar concentrations in a range
of tumor lines. In a genomewide screen for shRNAs that enhance
STF-118804 activity, shRNA against nicotinamide
phosphoribosyl transferase (NamPRT;
increased STF-118804 sensitivity compared with shRNAs against other targets. In
vitro, STF-118804 inhibited NAMPT activity. Next steps include
optimization and preclinical development of STF-118804 for hematological
Published online Nov. 21, 2013
Stanford University has pending patents on STF-118804; University
of California has filed patents on use of its shRNA
screening platform for target identification; both sets of patents available
Mattheny, C.J. et al.
Chem. Biol.; published online Oct. 31, 2013;
Contact: Michael L. Cleary, Stanford University School of
Medicine, Stanford, Calif.
All contents Copyright © 1993-2015 BioCentury Publications, Inc. ALL RIGHTS RESERVED. All use of this Web Site and its contents is governed by the BioCentury User Agreement
. The contents of this Web Site are protected under U.S. and foreign copyright and intellectual property laws, and no part of this Web Site or its contents may be photocopied, reproduced or retransmitted in any form without the written consent of BioCentury, which may be requested from Reprints/Permissions
. BioCentury®; The Bernstein Report on BioBusiness™; The BioCentury 100™; The Clear Route to ROI™; Because Real Intelligence is Hard to Find™; BCIQ™; and BioPharma's Knowledge Center™; are trademarks of BioCentury Publications, Inc., P.O. Box 1246, San Carlos, CA 94070. SciBX® and SciBX: Science-Business eXchange® are trademarks of Nature America, Inc. that are jointly used by BioCentury Publications, Inc. and Nature America, Inc.
Thanks for purchasing this article.
Please bookmark this url for future reference.
We have also sent you a confirming e-mail with a receipt and this same url for your
close [ X ]