Thursday, October 3, 2013
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Assays & screens
Assay for single-cell DNA
An assay for single-cell
DNA methylation analysis could be useful for understanding the heterogeneity
of epigenetic modifications in diseased tissue. The assay system combines
methylation-sensitive restriction digestion and multiplex, quantitative,
real-time PCR in a microfluidic device. The assay showed that early stage
mouse embryo cells with a deficiency in the scaffolding protein tripartite
motif containing 28 (Trim28)
exhibited variable degrees of hypomethylation at all tested genes. Next steps
include developing a complete set of assays to monitor methylation status at
all genes and adapting the assay for use with human cells.
Published online Oct. 3, 2013
status not applicable
Lorthongpanich, C. et
al. Science; published online Sept. 6, 2013;
Contact: Daniel M. Messerschmidt, Agency for Science,
Technology and Research (A*STAR), Singapore
Contact: William F. Burkholder, Nanyang Technological
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